Top Movie: HT 1080 cells invading through an ECM and exhibiting a mesenchymal phenotype as captured by the IncuCyte live-cell imaging system, time duration ~ 24 hours.

Bottom Movie: HT 1080 cells migrating on plastic, time duration ~ 8 hours.

The amount of invasion or migration is quantified by integrated algorithms on the IncuCyte. The Essen CellPlayer Invasion assay provides the ability to simultaneously observe both phenotypes on the same plate.