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IncuCyte Applications Proliferation |
Using IncuCyte for Monitoring ProliferationClick to view HD movie of proliferating CHO cells
Shown is a High Definition (HD) image of CHO cells. Also shown is growth curve plotting confluence vs. time at 3 hour intervals. Each data point is a composite of several vessel images at that time point.
Integrated object counting software in IncuCyte showing a.) original image b.) segmentation c.) image refinement and d.) object filtering capability. Images shown are from MCF7 cells expressing a nuclear GFP label. In contrast to traditional proliferation assays, IncuCyte can be used to measure cell proliferation kinetically, taking advantage of the fact that the cells do not have to be removed from the incubator and relying on automated image collection protocols to measure the same cells at different time points. These kinetic assays can be run in a labeled or non-labeled fashion and integrated software metrics provide temporal read-outs of proliferation vs. time. Non-labeled imaging is greatly enhanced using our proprietary HD Imaging mode which reduces meniscus artifacts, enabling high quality phase contrast images even in 96 and 384-well microplates. KEY FEATURES
DESCRIPTIONProliferation: IncuCyte can be used to follow kinetic cell proliferation in a number of ways. Using our integrated confluence algorithm as a surrogate for cell number, researchers can track the effects of drugs on cell proliferation without labeling. When absolute cell count is important, live-cell dyes such as Vybrant® DyeCycleTM Green (Invitrogen) or GFP labels in conjunction with our integrated Object Counting Software Module provides a direct readout of cell number vs. time.
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