Essen BioScience CellPlayerTM Cell Invasion Assay 
>96-Well IncuCyte microplate graph of alternating columns of HT 1080 cells plotting 2D migration on collagen I (odd columns) or 3D Invasion through collagen I (even columns) vs. time. Second plot is combined plate temporal statistics (mean, CV at each time point) demonstrating a CV=1.6% (n=48) for migration and CV=3.9% (n=48) for invasion at the chosen time point.
Above 96-well IncuCyte microplate graph demonstrating different amounts and rates of invasion of HT 1080 (col1-4), MDA-MB-231 (col 5-8) and MCF-7 (col 9-12) for increasing (top to bottom) concentrations of ECM.
KEY FEATURES
- How do we do it?: CellPlayer Invasion Protocol
- Reproducible Pharmacology: This assay allows researchers to assess the effects of pharmacological agents in the presence of different extra-cellular matrix components Click for Blebbistatin - GM6001 Pharmacology
- Follow Cell Morphology: IncuCyte images are automatically acquired at pre-programmed time points and assembled into time-lapse movies for convenient viewing. These images allow users to observe cell morphology changes associated with 3D invasive or 2D migration phenotypes Click for Cell Invasion Movie
- Directly Compare Migration and Invasion: Researchers can follow 2D migration and 3D invasion of different concentrations of ECM on the same plate and during the same time course. This eliminates running separate experiments as "migration" controls when studying invasion Example: 2D Migration and 3D Invasion on Same Plate
- Label-free: Based on our HD Imaging technique, quantitation of cellular invasion does not require a cell labeling step.
- Kinetic read-out: The spatio-temporal format of the invasion assay allows investigators to follow both the rate and the extent of cell invasion for a given set of experimental variables. This feature can be used to explore time-dependent pharmacology, improve assay optimization (e.g. ECM concentration, cell density), and enhance assay sensitivity
Biologically Quality Controlled Reagents
Essen BioScience is proud to introduce a new line of quality controlled extracellular matrix (ECM) components which support our cell invasion assay. ECM components are formulated from real tissue, and as a consequence vary from prepration to preparation and supplier to supplier. We have validated specific lots of Becton Dickinson (BD) reagents to ensure reliable performance in the CellPlayer Invasion Assay system.
Cat.# 4449 CellPlayer Rat Tail Collagen 1 Data Sheet 
Cat.# 4450 CellPlayer MatrigelTM Matrix Data Sheet
Cat.# 4451 CellPlayer Growth Factor Reduced (GFR) MatrigelTM Matrix Data Sheet Where to order: Contact
Matrigel is a registered trademark of Becton Dickinson Corporation
CELL INVASION APPLICATION NOTE
CellPlayer 96-Well Cell Invasion Application Note
Cell Invasion Assay Services: Customers also have the option of using our Assay Services for assay development or drug screening. In this model, customers can take advantage of our experience in running cell invasion assays to quickly answer either proof-of-concept or drug screening questions. This frees up internal resources if outsourcing is preferred or provides an entry point for evaluating IncuCyte technology prior to making a purchase.
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