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IncuCyte Applications

Cell Culture QC

 

 

Using IncuCyte for Cell Culture QC

Cell Culture Quality Control

Click to view movie of proliferating CHO cells

Cell Culture Quality Control

Kinetic confluence curves demonstrating the effects of initial cell seeding density on long-term confluence

Cell Culture Quality Control

IncuCyte provides a simple, non-invasive, time-lapse method of archiving and quantifying cell growth inside your cell culture incubator.

KEY FEATURES

  • Gather time-lapse imagery of proliferating cells without using labels and without removing cells from your incubator
  • Use built-in kinetic metrics such as monolayer confluence to optimize when to split cells and when to run assays
  • Excellent for QC in microplates, proprietary HD Imaging Mode removes meniscus artifacts in 96-well and 384-well plates
  • Eliminate human interpretation by using objective algorithms to characterize cell growth
  • System is capable of viewing large areas of cell culture vessels increasing the accuracy of the assessment
  • All temporal metrics are validated by images and movies

 

DESCRIPTION

Cell Culture: There are many different factors which can adversely affect cell culture and cell-based assays including; a.) human error, subjectivity and inconsistency b.) environment c.) reagent and lab ware variability and d.) the time-dependent nature of biology itself.

IncuCyte can be used to standardize cell culture procedures, document growth and morphology over time, isolate variables and optimize cell-based assay performance.

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