Live-Cell Analysis of Cancer Cell Metabolism

Metabolic reprogramming is a hallmark of cancer, and it supports the increased energetic, biosynthetic, and redox demands associated with tumor development and progression. Therapeutic approaches exploiting differential metabolic requirements of tumor cells are in under investigation and in clinical trials. At the same time, mitochondrial toxicity has been implicated in clinical trial attrition and black box warnings, making predictive screening an important step in the drug development process.

Standard approaches to monitoring compound-induced metabolic perturbations rely on endpoint assays that provide limited kinetic information and lack cell-specific data in co-culture models. Live-cell analysis holds promise for greater physiological relevance and insight by providing the necessary tools to study dynamic cells changes in translational models.

This webinar, presented by Cicely Schramm, discusses:

• Use of a live-cell approach to image and analyze intracellular ATP levels of cells in both mono and co-culture models, using the Incucyte® SX5 System

• The value of live-cell ATP analysis to evaluate compound selectivity for development of novel therapeutics

• Examination of stromal cell-mediated resistance in clinically relevant models

• How direct, live-cell measurement of ATP can be implemented to screen for mitochondrial toxicity of drug candidates

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