IncuCyte® NeuroTrack Software Module (Cat No 9600-0010)

Automated phase contrast image segmentation of neurites

Automated phase contrast image segmentation of neurites (yellow) and cell body clusters (blue) in rat cortical neurones using Neurotrack and the IncuCyte® live-cell analysis system.

The IncuCyte® Neurotrack software module allows users to automatically process IncuCyte® images and to analyze neurite dynamics with or without labels in living cells. The algorithm masks and quantifies each image and returns neuronal cell metrics including neurite length, branch points, cell body area and number of cell clusters. These metrics are visualized in full timecourse plots for each well in 96- and 384-well plates. Analyses can be performed directly on phase contrast images obviating the need for cell labels, or on fluorescent images using non-perturbing probes as may be required in co-culture systems.

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Incucyte® Live Cell Analysis System

Gain insights into biological processes of cells in real time via non-perturbing quantitative analysis

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IncuCyte® NeuroPrime Cell Kit (Cat No 4585)

The first commercially available cryopreserved neuronal/astrocyte co-culture kit designed for long term, kinetic live cell imaging and measurement of neurite dynamics. Each cell kit contains cryopreserved cell vials of rat forebrain neurons and rat astrocytes, and a vial of the non-perturbing neuronal labeling reagent, IncuCyte® NeuroLight Red. A fully validated protocol for thawing, labeling and measuring neurite outgrowth in a 96-well format using the IncuCyte® Live-Cell Analysis System is also included.

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IncuCyte® NeuroLight Red Lentivirus - Synapsin Promoter (Cat No 4584)

One vial of lentivirus reagent (0.45mL) suitable for transducing a variety of neuronal cell types including cell lines, primary and stem cell-derived cultures. The IncuCyte® NeuroLight Red lentivirus encodes a red fluorescent protein (mKate2) driven off a synapsin promoter to strengthen neuronal expression and minimize non-neuronal crossover.

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IncuCyte® ZOOM 20X Objective (Cat No 4465)

20x Microscope Objective for IncuCyte® ZOOM. Required for high spatial resolution cell imaging

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Neuro-2a IncuCyte® NucLight Green Cells (Cat No 4511)

One cryopreserved vial of Neuro-2a NucLight Green (nuclear-labelled GFP) expressing cells at a density of 2x10^6 cells/vial.

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Neuro-2a IncuCyte® NucLight Red Cells (Cat No 4512)

One cryopreserved vial of Neuro-2a NucLight Red (nuclear-labbelled RFP) expressing cells at a density of 2x10^6 cells/vial.

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Neurite Dynamics - Label-free

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Neuronal Co-Culture - Fluorescence

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