IncuCyte® ClearView 96-Well Chemotaxis Plate

Combine the optical clarity of microfluidics devices with the throughput of transmembrane assays. Each well of a ClearView plate provides an optically clear surface for label-free imaging and analysis of chemotactic cell migration using the IncuCyte® Live-Cell Analysis System.


Overview of the ClearView Chemotaxis Plate design

See what's in the box

The IncuCyte® ClearView Chemotaxis Plate combines the throughput of transmembrane assays with the optical clarity of microfluidics devices. Each well of a ClearView plate provides an optically clear surface for label-free imaging and analysis of chemotactic cell migration using the IncuCyte® live-cell analysis system.

The ClearView membrane contains 96 laser etched pores with a diameter of 8µm. Cells are added to the upper chamber and chemoattractant to the lower reservoir plate. Chemotactic transmembrane migration is visually monitored within your incubator and automatically analyzed using the IncuCyte live-cell analysis system.

IncuCyte® ClearView Chemotaxis Plate

Plate composition

Each IncuCyte® ClearView Cell Migration Plate is composed of :

  • One plate lid (A)
  • One 96-well ClearView membrane insert (B)
  • One 96-well reservoir plate (C)

Key advantages


Visualize every cell

  • The specially designed ClearView membrane enables you to monitor every cell throughout your experiment in real time – gain deep phenotypic insight into cell movement and morphology

The ClearView membrane enables label-free visualization and quantitation of chemotaxis

Monitor every cell in your experiment. The ClearView membrane enables label-free visualization and quantitation of chemotaxis using IncuCyte® system. Each membrane contains 96 precision engineered holes in a regular grid array. Three pores are highlighted in yellow.


Low cell usage ideal for rare, expensive and primary cell populations

  • Use 10- to 100-fold fewer cells than standard transmembrane (e.g. Boyden Chamber) approaches
Cell usage comparison for the ClearView Plate and Corning® Transwell® system

Lower cell usage. Cell usage comparison for the ClearView Plate and Corning® Transwell® system. Jurkat T cell chemotaxis toward SDFα-1 was measured in both systems (2,000 to 20,000 cells/well); however, no signal was observed with the Transwell® plate. A minimum of 150,000 cells/well was required to observe chemotactic migration using the Corning® Transwell® system.

 

Biologically relevant surface-mediated migration

  • Cells must migrate across a biologically relevant surface towards the chemoattractant due to the low pore density of the specially designed ClearView membrane (pore density <2% that of Boyden chamber transwells)
  • The ClearView plate has been validated with a wide range of biomatrix coatings, cell types and chemoattractants

Time-lapse images of a Jurkat T cell

Cells must interact with substrate to reach a pore. Time-lapse images of a Jurkat T cell (outlined in white) migrating across a Matrigel® coating towards a pore leading to the chemoattractant SDF-1α (orange circle). White arrows indicate the direction of Jurkat movement in each frame. In the final image the Jurkat T cell has entered the pore and moved to the lower chamber.


Stable and sustained gradients

  • Chemotactic gradients are stable and maintained over 72 hours

Gradient stability was monitored over 72 hours using an Alexa Fluor® 594 dextran

Long-term stable gradients compared to traditional Boyden chamber transwells. (A) Gradient stability was monitored over 72 hours using an Alexa Fluor® 594 dextran (10 kDa). After 4 hours a rapid loss in gradient was measured in the Corning® Transwell® system. In contrast, >80% of the gradient was maintained in the IncuCyte® ClearView plate at 72 hours. (B) FBS gradients pre-establised in a ClearView plate for 24, 48, and 72 hours induced equivalent rates of directional migration in HT1080 cells indicating long-term and stable gradients across the ClearView membrane. Rates were slightly faster than for cells migrating towards gradients that had not been pre-established. This is likely a result of increased cell health when serum starved cells are plated in established gradients.


Compatible with standard automation and liquid dispensing devices

  • 96-well format suitable for screening workflows
  • Compliant with SBS microtiter plate requirements

Comparison of the ClearView Chemotaxis Plate with existing Boyden chamber transwell and microfluidic approaches

ClearView Boyden Chambers Microfluidic Devices
Visually observe cell
movement and morphology
Label-free analysis
Pore density Low High N/A
Stable chemotactic gradient
Kinetic read-out
Low cell usage (< 5,000 per well)
Surface interactions– Integrin sensitivity
96-well throughput or higher
Workflow: set up and walk away
Automation friendly

Ordering Information

Catalog No. Product name Qty
4582 IncuCyte® ClearView 96-Well Chemotaxis Plates 1 plate
4599 IncuCyte® ClearView 96-Well Chmotaxis Plates – Case of 50 Plates 50 plates
4600 IncuCyte® ClearView Reservoir Plate – 10 Pack 10 plates
4601 IncuCyte® ClearView Reservoir Plate – Case of 100 Plates 100 plates

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Incucyte® Live Cell Analysis System

Gain insights into biological processes of cells in real time via non-perturbing quantitative analysis

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IncuCyte® Chemotaxis Software (Cat No 9600-0015)

An add-on software module for the IncuCyte ® Live-Cell Analysis System. Analyze label-free and fluorescently-labelled chemotactic cell migration images acquired using the ClearView Cell Migration Plate.

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Chemotaxis Cell Migration & Invasion

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