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The most common in vitro methods for immunologists to analyze cells of the immune system are flow cytometry, PCR and various forms of ELISA. Together, these enable quantification of different cell populations at the molecular and functional level (‘immuno-phenotyping’) and measurement of immune responses, for example, cytokine release. While these platforms are extremely powerful, they provide little insight into morphology and the spatial interactions of cells. Moreover, as ‘end-point’ measures, they are not well suited to report on the dynamic changes in biology over time.

Learn long-term live cell imaging for high-content applications & learn about the new developments in image acquisition that combine live cell incubation and imaging with high-content screening approaches.

Learn how live-cell analysis provides an enabling toolkit of assays and reagents for studying the interplay between cancer and immune cells, in real-time